| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.214294 |
| Chromosome: | chromosome 7 |
| Location: | 4923155 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g346418 | (1 of 2) PTHR13271//PTHR13271:SF30 - UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGAGGGGCAGGTGGGGAGGGAGAGGCAG |
| Internal bar code: | TAGTGCGGCGTGGACCGTTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 782 |
| LEAP-Seq percent confirming: | 74.3678 |
| LEAP-Seq n confirming: | 647 |
| LEAP-Seq n nonconfirming: | 223 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTTCTTCTTCTTGTCCCC |
| Suggested primer 2: | GCGCTTACCGGTTTACACAT |