| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.214301 |
| Chromosome: | chromosome 16 |
| Location: | 5872508 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677550 | CYA15 | (1 of 8) PF00211//PF01547 - Adenylate and Guanylate cyclase catalytic domain (Guanylate_cyc) // Bacterial extracellular solute-binding protein (SBP_bac_1); Solute-binding protein-like adenylate cyclase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGGAGCGGAGGTCGCGTCCGAAGGCAGG |
| Internal bar code: | GCAAGATACGCGACGACAGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 214 |
| LEAP-Seq percent confirming: | 91.1765 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTACTGGGAGCAGTGATG |
| Suggested primer 2: | CCCTCGTGCCACTTAATGTT |