Insertion junction: LMJ.RY0402.214315_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.214315
Chromosome:chromosome 16
Location:4053768
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g670000 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGGACATGATAGGATGCGCATGTGCGTTT
Internal bar code:TGTTGGCAGATGCCGACATGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1035
LEAP-Seq percent confirming:97.1168
LEAP-Seq n confirming:10880
LEAP-Seq n nonconfirming:323
LEAP-Seq n unique pos:48

Suggested primers for confirmation by PCR

Suggested primer 1:CTCGGTTTGAGTTCCTGCTC
Suggested primer 2:CGGTTTGGGTCTTTAGGTGA