Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.214326 |
Chromosome: | chromosome 1 |
Location: | 5295501 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre01.g037000 | SCPL49,CPY | Serine carboxypeptidase; (1 of 2) PTHR11802//PTHR11802:SF72 - SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTGCTGCTTGGACTTGCGGTCGCAGCAG |
Internal bar code: | GCCGCTTAGGTGATCTTAAAGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 727 |
LEAP-Seq percent confirming: | 99.7852 |
LEAP-Seq n confirming: | 6503 |
LEAP-Seq n nonconfirming: | 14 |
LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCTACGTGCTGCTGCAGTCT |
Suggested primer 2: | CGTGTTTCCCTTTACCCAGA |