| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.214331 |
| Chromosome: | chromosome 9 |
| Location: | 4331607 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394917 | (1 of 4) PTHR16255//PTHR16255:SF4 - UNCHARACTERIZED // SPORULATION PROTEIN RMD8 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACATGTTGGGGGCGCTGACAGCGGCGA |
| Internal bar code: | TGCGGTTACACAGGTGGGTCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 754 |
| LEAP-Seq percent confirming: | 57.3276 |
| LEAP-Seq n confirming: | 2926 |
| LEAP-Seq n nonconfirming: | 2178 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCACTCTGCACTCATCAAA |
| Suggested primer 2: | CGCCGTAGTCGAAGAAGAAC |