| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.214335 |
| Chromosome: | chromosome 17 |
| Location: | 5172579 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g735500 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGCCAGCGGCCGGGTTGAATGGGTACTA |
| Internal bar code: | GTTCGAGATTCAAACATCTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 385 |
| LEAP-Seq percent confirming: | 99.6239 |
| LEAP-Seq n confirming: | 24106 |
| LEAP-Seq n nonconfirming: | 91 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGATGGCAAAGGTCAGAT |
| Suggested primer 2: | GCGCTCATAGGCTTTACGAC |