Insertion junction: LMJ.RY0402.214341_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.214341
Chromosome:chromosome 16
Location:5960700
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g676850 (1 of 14) IPR003593//IPR003959//IPR027417 - AAA+ ATPase domain // ATPase, AAA-type, core // P-loop containing nucleoside triphosphate hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCTCTCACGTGCCCATGGCTCTCAGCGCC
Internal bar code:ACCGCCCCGGCCCCTAGGAACC

Confirmation - LEAP-Seq

LEAP-Seq distance:407
LEAP-Seq percent confirming:99.278
LEAP-Seq n confirming:2750
LEAP-Seq n nonconfirming:20
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:TTCTGTCCAGTTTGTCTGCG
Suggested primer 2:AGCACACCACCATGCAATAA