| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.214342 |
| Chromosome: | chromosome 2 |
| Location: | 6060862 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g112701 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTAAAGTAGGGCGCGATGCTTCGGCGGGC |
| Internal bar code: | GACCACAACGGTAGGAACACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 697 |
| LEAP-Seq percent confirming: | 99.5908 |
| LEAP-Seq n confirming: | 8275 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGCAGAGCTTTCAGGAGT |
| Suggested primer 2: | TTTGCGACTGTTCTGGAGTG |