| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.214369 |
| Chromosome: | chromosome 3 |
| Location: | 6153831 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g192000 | SEC13L,SEH1 | COP-II coat subunit, nucleoporin; (1 of 1) K14299 - nucleoporin SEH1 (SEH1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGTCAATGCCTTCTGCGGCGCCGCAGAC |
| Internal bar code: | ACATCGGATCCCGACGAGGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 504 |
| LEAP-Seq percent confirming: | 99.1091 |
| LEAP-Seq n confirming: | 445 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCCCCCTCACCTTGACTT |
| Suggested primer 2: | GGCGTTGTCCGTGTCTTTAT |