| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.214371 |
| Chromosome: | chromosome 6 |
| Location: | 1250509 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g257950 | AST4 | (1 of 1) K14455 - aspartate aminotransferase, mitochondrial (GOT2); aspartate aminotransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCAAGAACATGGGGCTGTACGGCCAGCGC |
| Internal bar code: | CCCACTGGTTTGCAATCTCAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 487 |
| LEAP-Seq percent confirming: | 99.7972 |
| LEAP-Seq n confirming: | 492 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTACTACAAGGCCGAGAC |
| Suggested primer 2: | GCCCAAACTAAACCAAACCA |