| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.214386 |
| Chromosome: | chromosome 6 |
| Location: | 3912552 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278183 | (1 of 1) PTHR23054//PTHR23054:SF14 - UNCHARACTERIZED // PROTEIN Y45F10A.7, ISOFORM A | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGAGTGTGACCGTGTGTAAATTTGTGTGA |
| Internal bar code: | AGGGGATTAATAGCGGGCGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 980 |
| LEAP-Seq percent confirming: | 97.03 |
| LEAP-Seq n confirming: | 6926 |
| LEAP-Seq n nonconfirming: | 212 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACGCACAACTACTCCGTA |
| Suggested primer 2: | AACCCAGTCTCACCGATGTC |