| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.214397 |
| Chromosome: | chromosome 17 |
| Location: | 3997959 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g728900 | FSA1,TAL3 | (1 of 1) PTHR10683//PTHR10683:SF2 - TRANSALDOLASE // SUBFAMILY NOT NAMED; Transaldolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGATCATGGAGGGGGGCTGGGCCCGATCG |
| Internal bar code: | TCAACAAGGTTTAATTCCGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 708 |
| LEAP-Seq percent confirming: | 99.503 |
| LEAP-Seq n confirming: | 5806 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTAGGAATGCCTGGACAGA |
| Suggested primer 2: | ATGGCTGTTTCCCTTGACAC |