| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.214405 |
| Chromosome: | chromosome 15 |
| Location: | 1179903 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g641150 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGAGCCGGCAGCGGCACAGGCGGCGCCG |
| Internal bar code: | GGAGTGTAGACTCCGTGACATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 85 |
| LEAP-Seq percent confirming: | 93.4537 |
| LEAP-Seq n confirming: | 414 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGCGGTGGTGGTACTAGG |
| Suggested primer 2: | ACAAAGGGCACAATGACACA |