| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.214418 |
| Chromosome: | chromosome 4 |
| Location: | 3656972 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g228800 | (1 of 19) K08850 - aurora kinase, other [EC:2.7.11.1] (AURKX) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCCCCGACGTAGTGCGGTCCGGGCTGGG |
| Internal bar code: | CCTTCGCCGCTCACAACAGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1043 |
| LEAP-Seq percent confirming: | 99.2938 |
| LEAP-Seq n confirming: | 8014 |
| LEAP-Seq n nonconfirming: | 57 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGAATTGACAACAAGGC |
| Suggested primer 2: | TCACGTCCTCTCGATGTCAG |