| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.214430 |
| Chromosome: | chromosome 17 |
| Location: | 4197986 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g730150 | (1 of 26) IPR029058//IPR029059 - Alpha/Beta hydrolase fold // Alpha/beta hydrolase fold-5 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCACACTCGCACAGGCGCTGACGTGGATC |
| Internal bar code: | CGCGCATGTAAGCCTCGGGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 368 |
| LEAP-Seq percent confirming: | 96.5035 |
| LEAP-Seq n confirming: | 2208 |
| LEAP-Seq n nonconfirming: | 80 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTAGATGAAGTTGGCGAGC |
| Suggested primer 2: | CATCCCCGATATCCCCTTAT |