| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.214448 |
| Chromosome: | chromosome 4 |
| Location: | 3330029 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g227251 | (1 of 1) IPR006626//IPR008752//IPR011050//IPR029018 - Parallel beta-helix repeat // Peptidase M11, gametolysin // Pectin lyase fold/virulence factor // Chitobiase/beta-hexosaminidase domain 2-like | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACTGGGATTCGGGACCGTTGCGTCCCT |
| Internal bar code: | CTAAGCGAGCCGCGGTTTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 443 |
| LEAP-Seq percent confirming: | 94.9612 |
| LEAP-Seq n confirming: | 3185 |
| LEAP-Seq n nonconfirming: | 169 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAAAGTGCCAAACTGGTGC |
| Suggested primer 2: | CCTACTCGGACCTCAAGCTG |