| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.214478 |
| Chromosome: | chromosome 12 |
| Location: | 572295 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g492700 | FAS1 | (1 of 10) PTHR10900 - PERIOSTIN-RELATED; Fasciclin-like protein 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGGTGTTGCGCAACCGTCAATCCGTCAC |
| Internal bar code: | GCGCGCTTCCGTTATATCAACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 624 |
| LEAP-Seq percent confirming: | 99.4498 |
| LEAP-Seq n confirming: | 1446 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTATGTACAGCCAGCGTGC |
| Suggested primer 2: | TCGTGAGTGTTGAGTCGGAG |