| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.214483 |
| Chromosome: | chromosome 10 |
| Location: | 4524689 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g452150 | (1 of 100) IPR001810 - F-box domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCCCTTGTCCCGCCTGGCTTGGTCCTCC |
| Internal bar code: | AGGGTAATGGGAGCTTGTTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1121 |
| LEAP-Seq percent confirming: | 98.7263 |
| LEAP-Seq n confirming: | 7441 |
| LEAP-Seq n nonconfirming: | 96 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGAACGGCAGAAAGTACAA |
| Suggested primer 2: | GGAGGAGAAGGAGGAGGAGA |