Insertion junction: LMJ.RY0402.214500_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.214500
Chromosome:chromosome 4
Location:2580151
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g222550 (1 of 43) IPR011989 - Armadillo-like helical intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATCATTCTAGATCGTATGACCGTGTGCTG
Internal bar code:CCTGGTCGTCCCCTCCCGCTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:424
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCTTGGCCATACCTGTGT
Suggested primer 2:AACTCTTCAATCTGCCGCAT