Insertion junction: LMJ.RY0402.214514_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.214514
Chromosome:chromosome 9
Location:5168450
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g399141 PHT4B,PHT4-2 Sodium-dependent phosphate transporter, major facilitator superfamily; (1 of 1) PTHR11662:SF282 - ANION TRANSPORTER 5-RELATED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGCTCATTCGGTTGCTCGCTCGCTTGTA
Internal bar code:AAGAATAACCGTCGGACATAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:259
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GCGCAAAGAAGTTGAAGACC
Suggested primer 2:GTAGGGTGATGCATGACGTG