| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.214530 |
| Chromosome: | chromosome 4 |
| Location: | 1949746 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g218450 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTCCTTGTAGACGCCGTACTCCGTCTGA |
| Internal bar code: | CCGCCAGACGCCATTTGTGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 757 |
| LEAP-Seq percent confirming: | 99.8236 |
| LEAP-Seq n confirming: | 1698 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCTCACGTGCATCAGGTC |
| Suggested primer 2: | ATAAGTTGCTTGCTCGCGTT |