| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.214553 |
| Chromosome: | chromosome 16 |
| Location: | 5933513 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677000 | HSP70E,GEX27 | (1 of 1) K09489 - heat shock 70kDa protein 4 (HSPA4); Heat shock protein 70E | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCTCAAAGTAATGAATGTCCGCTTTGCC |
| Internal bar code: | GGGATCACCCGCTGAACGGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 608 |
| LEAP-Seq percent confirming: | 86.5986 |
| LEAP-Seq n confirming: | 3903 |
| LEAP-Seq n nonconfirming: | 604 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCCCTGAGCTCTTTAGGC |
| Suggested primer 2: | GCCGCACTTTAGCTTGTTTC |