| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.214559 |
| Chromosome: | chromosome 13 |
| Location: | 1540390 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g572900 | THO3 | (1 of 1) 3.4.24.70 - Oligopeptidase A; Oligoendopeptidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGCACCAGCACTCGGCATCATCCCTTCC |
| Internal bar code: | GCTTTGTCCAATTCCTAATGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 356 |
| LEAP-Seq percent confirming: | 99.6328 |
| LEAP-Seq n confirming: | 2713 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGAGGTGAGGTGAGGTGGT |
| Suggested primer 2: | CCAAGTTCAGCAACAACGTG |