| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.214569 |
| Chromosome: | chromosome 1 |
| Location: | 4296632 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g028900 | (1 of 752) IPR027417 - P-loop containing nucleoside triphosphate hydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGGAAAGCTGCTGCTATGCTGGCAACGC |
| Internal bar code: | GGTTTGTGCGGGGGATCCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1084 |
| LEAP-Seq percent confirming: | 93.7441 |
| LEAP-Seq n confirming: | 11898 |
| LEAP-Seq n nonconfirming: | 794 |
| LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCCCATTCTCTACCCACT |
| Suggested primer 2: | CAGATAGGTGGTGCCATGTG |