Insertion junction: LMJ.RY0402.214571_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.214571
Chromosome:chromosome 3
Location:7344581
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g206550 CPLD2,PGP5 (1 of 2) PTHR18901//PTHR18901:SF9 - 2-DEOXYGLUCOSE-6-PHOSPHATE PHOSPHATASE 2 // SUBFAMILY NOT NAMED; conserved expressed protein with hydrolase motif intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTGCCACTCACGTCATGCCCGCGGCCTTG
Internal bar code:TGGCTCATTATAGCCTTCGTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:903
LEAP-Seq percent confirming:99.675
LEAP-Seq n confirming:2760
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:AAGGCAAGTCAGTGCTTCGT
Suggested primer 2:GCTTCTTGCACAGAACCCTC