| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.214576 |
| Chromosome: | chromosome 3 |
| Location: | 8702252 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g204241 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAAGGGGGGAGGGAAGTGCGAAGGGAGGG |
| Internal bar code: | TTATCCCGCAACTCTGTGGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 309 |
| LEAP-Seq percent confirming: | 82.1123 |
| LEAP-Seq n confirming: | 40350 |
| LEAP-Seq n nonconfirming: | 8790 |
| LEAP-Seq n unique pos: | 112 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGCAGGTAAGGAGGAGAG |
| Suggested primer 2: | TCGAGCACACGGATAAACAC |