| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.214589 |
| Chromosome: | chromosome 12 |
| Location: | 4113815 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g518000 | SCA2,SECA2 | (1 of 1) PF01043//PF07517 - SecA preprotein cross-linking domain (SecA_PP_bind) // SecA DEAD-like domain (SecA_DEAD); Chloroplast-associated SecA protein | CDS|intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGTTCGGCCAGCGCAGATCGGCATGGCA |
| Internal bar code: | GGTTGTCGTGTTCCATCCACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 881 |
| LEAP-Seq percent confirming: | 97.0165 |
| LEAP-Seq n confirming: | 5528 |
| LEAP-Seq n nonconfirming: | 170 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTTGAAGTGGCTTCTGGG |
| Suggested primer 2: | CTTCGACTGACCAAAGAGGC |