| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.214591 |
| Chromosome: | chromosome 7 |
| Location: | 1469136 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g323500 | (1 of 1) PF08609 - Nucleotide exchange factor Fes1 (Fes1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGTTGGGGATGGCGACGCCGGGGGTAGT |
| Internal bar code: | GTATCGGCAGGGCAGCTAAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 833 |
| LEAP-Seq percent confirming: | 98.9002 |
| LEAP-Seq n confirming: | 1259 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTTCTCCCAGTCTGTTGC |
| Suggested primer 2: | GTGTCCATTTTGTGCGTGAC |