| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.214595 |
| Chromosome: | chromosome 10 |
| Location: | 1434847 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g428433 | FDG2 | Formamidopyrimidine-DNA glycosylase; (1 of 1) 3.2.2.23 - DNA-formamidopyrimidine glycosylase / Formamidopyrimidine-DNA glycosylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCTACGGCGCGCGTGTGTTTGCCAATCA |
| Internal bar code: | GGTTCTGCCGCGAGCCCCATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 608 |
| LEAP-Seq percent confirming: | 56.928 |
| LEAP-Seq n confirming: | 3899 |
| LEAP-Seq n nonconfirming: | 2950 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGTCAGGGTTGGTGAACT |
| Suggested primer 2: | GCTTTTGATAGACGCAAGGC |