| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.214601 |
| Chromosome: | chromosome 9 |
| Location: | 756656 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g402402 | (1 of 80) IPR003882 - Pistil-specific extensin-like protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAGCTCAGTCAAAGTGGGCAGGCACGGCT |
| Internal bar code: | GTGCTTCTAGACATATAATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 396 |
| LEAP-Seq percent confirming: | 99.6436 |
| LEAP-Seq n confirming: | 6150 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACGCTAGTGGTTTGGGGA |
| Suggested primer 2: | GTGGTTGTGTCAGGTTGTGG |