Insertion junction: LMJ.RY0402.214612_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.214612
Chromosome:chromosome 1
Location:1653918
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g008650 CDK1 Cyclin-dependent kinase; (1 of 12) 2.7.11.22//2.7.11.23 - Cyclin-dependent kinase / Cdk-activating protein kinase // [RNA-polymerase]-subunit kinase / CTD kinase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGAGGGGGGGGGATACCGGCCCAGTCGCG
Internal bar code:GAACGTCTGGGGGGGATCCCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:1074
LEAP-Seq percent confirming:99.8105
LEAP-Seq n confirming:3687
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CTCGTACAGGACTGGTGGGT
Suggested primer 2:TGGTGGTAAGGTTTGGTGGT