| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.214614 |
| Chromosome: | chromosome 16 |
| Location: | 4461219 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g670750 | (1 of 1) IPR012312//IPR012827 - Haemerythrin-like // Haemerythrin, metal-binding domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGGGTGTTTACTGCATGTGCACAGGGCA |
| Internal bar code: | TCAGGGCCGCACGTGATTTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 880 |
| LEAP-Seq percent confirming: | 99.4483 |
| LEAP-Seq n confirming: | 9194 |
| LEAP-Seq n nonconfirming: | 51 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATCTGTACCACCACATCG |
| Suggested primer 2: | TTTCAATGGTCAGGGAGGAG |