| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.214629 |
| Chromosome: | chromosome 2 |
| Location: | 2368006 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g091000 | (1 of 1) PF05346 - Eukaryotic membrane protein family (DUF747) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTCCACGGCTTACCTCATGCCCGGAGGT |
| Internal bar code: | TGGTTGGGAGTGCTTCAGGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 496 |
| LEAP-Seq percent confirming: | 99.4366 |
| LEAP-Seq n confirming: | 1412 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCTTGCCTAACGGGTCAC |
| Suggested primer 2: | GTACAGCGTACGGCAAGGAT |