| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.214633 |
| Chromosome: | chromosome 3 |
| Location: | 5668083 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g187550 | (1 of 1) PF04858 - TH1 protein (TH1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGCCCTCTGATGCACACCGCCCCCGCGG |
| Internal bar code: | ACGGACCTGTTTGGCACGAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 380 |
| LEAP-Seq percent confirming: | 98.9377 |
| LEAP-Seq n confirming: | 4191 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTATGGCGAAGTTGAGCAG |
| Suggested primer 2: | CGGAAATGGTGAGCAAGTTT |