| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.214634 |
| Chromosome: | chromosome 16 |
| Location: | 3500168 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g675602 | (1 of 1) K12669 - oligosaccharyltransferase complex subunit gamma (OST3, OST6) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCGGGATCACCTGTGCCATCGCCCGAAA |
| Internal bar code: | TCTGGCTCCCTATTGTCTCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 789 |
| LEAP-Seq percent confirming: | 99.8357 |
| LEAP-Seq n confirming: | 6076 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAATAACCGTATCGCACCA |
| Suggested primer 2: | CGAACTTTCGCAAAGTGACA |