| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.214643 |
| Chromosome: | chromosome 12 |
| Location: | 2740676 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g503300 | (1 of 1) 4.1.1.21 - Phosphoribosylaminoimidazole carboxylase / AIR carboxylase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGAGCATTCGCAGGCGAGAATGTTTTAA |
| Internal bar code: | GCATTGGACTAAGGAGCCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 683 |
| LEAP-Seq percent confirming: | 91.0084 |
| LEAP-Seq n confirming: | 1083 |
| LEAP-Seq n nonconfirming: | 107 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCCTTTTTCCTCCTACCC |
| Suggested primer 2: | TTGCTTACACGCAGGAGATG |