Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.214643 |
Chromosome: | chromosome 12 |
Location: | 2740681 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g503300 | (1 of 1) 4.1.1.21 - Phosphoribosylaminoimidazole carboxylase / AIR carboxylase | 5'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGAAACGTGTGGGCGCCGTACGACGCGC |
Internal bar code: | GTATTCAGGCTGTCAAGGTTCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 928 |
LEAP-Seq percent confirming: | 99.5936 |
LEAP-Seq n confirming: | 5146 |
LEAP-Seq n nonconfirming: | 21 |
LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTGCTTACACGCAGGAGATG |
Suggested primer 2: | CAGCCTTTTTCCTCCTACCC |