| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.214664 |
| Chromosome: | chromosome 2 |
| Location: | 163843 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g074370 | CDPK8 | Calcium/calmodulin-dependent protein kinase; (1 of 18) K13412 - calcium-dependent protein kinase (CPK) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACCAGGACCCCCCACCCTTCGTCCACCA |
| Internal bar code: | TAGACCCCCTTGCGCATCATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 581 |
| LEAP-Seq percent confirming: | 99.1276 |
| LEAP-Seq n confirming: | 6363 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTCTTGTAGTCCGACCCT |
| Suggested primer 2: | GCCACAAACACAGAGCAGAA |