| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.214665 |
| Chromosome: | chromosome 12 |
| Location: | 566188 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g492750 | (1 of 5) 3.2.1.1 - Alpha-amylase / Glycogenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGTGCTGTGATGCCAATTGTGCAGGCT |
| Internal bar code: | TAATTGGCGTGGACTCCGAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 145 |
| LEAP-Seq percent confirming: | 98.5916 |
| LEAP-Seq n confirming: | 70 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGTCCTTCCAGTGCTGCT |
| Suggested primer 2: | GCACCTACCTTCTGTGCCTC |