| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.214680 |
| Chromosome: | chromosome 1 |
| Location: | 2137636 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g011600 | (1 of 1) PTHR23244//PTHR24202//PTHR24202:SF4 - KELCH REPEAT DOMAIN // FAMILY NOT NAMED // E3 UBIQUITIN-PROTEIN LIGASE MIB2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGGCATGCTCCCACCTGTCCAACTGTTG |
| Internal bar code: | ATGAAGGCTCGGGTTTACTCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 846 |
| LEAP-Seq percent confirming: | 99.493 |
| LEAP-Seq n confirming: | 10792 |
| LEAP-Seq n nonconfirming: | 55 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTTGCCTGTGGTTGGACT |
| Suggested primer 2: | CTAAAGACGCCAAGACCAGC |