| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.214697 |
| Chromosome: | chromosome 8 |
| Location: | 3348688 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g374150 | (1 of 11) IPR011146 - HIT-like domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGTGGGCCGAGGTGCGTGTGCGTCTGGT |
| Internal bar code: | GACACCACGCACGTACCGATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 216 |
| LEAP-Seq percent confirming: | 92.2148 |
| LEAP-Seq n confirming: | 687 |
| LEAP-Seq n nonconfirming: | 58 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATGCGATATTCTGGGACA |
| Suggested primer 2: | TCCCGGTAGTAGCAGACACC |