| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.214701 |
| Chromosome: | chromosome 14 |
| Location: | 1991321 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g621400 | (1 of 1) IPR001810//IPR025886 - F-box domain // Phloem protein 2-like | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTGGCAACCAGGACGCTTGGCGGCGGGT |
| Internal bar code: | AAATACTGAGATAATCGGTCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 950 |
| LEAP-Seq percent confirming: | 99.5609 |
| LEAP-Seq n confirming: | 4762 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCATTGGACCTCCCTATC |
| Suggested primer 2: | TCAACAAGTTTTGGCACAGC |