| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.214703 |
| Chromosome: | chromosome 3 |
| Location: | 3874327 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g171050 | GHL1 | (1 of 2) 3.2.1.21 - Beta-glucosidase / Gentobiase; Glycosyl hydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTTATTTGCCCCCTGCTGACCGGGCACG |
| Internal bar code: | ATTACAGCTACGCCATAGCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 313 |
| LEAP-Seq percent confirming: | 99.0215 |
| LEAP-Seq n confirming: | 506 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGAAAAGCGGTAAAGGATG |
| Suggested primer 2: | GCAAACCTAGGAGCAGATCG |