| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.214704 |
| Chromosome: | chromosome 8 |
| Location: | 260385 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358555 | (1 of 2) PTHR10625//PTHR10625:SF131 - HISTONE DEACETYLASE // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGGAGTCGAGGGTTGCGTGGCGTGCCGG |
| Internal bar code: | GCATCGAGGAACACCGCGCGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 905 |
| LEAP-Seq percent confirming: | 98.8654 |
| LEAP-Seq n confirming: | 9149 |
| LEAP-Seq n nonconfirming: | 105 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAAAAGACCCCTCGTTTCA |
| Suggested primer 2: | ATGTCGTCGTGTGATCGTGT |