| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.214705 |
| Chromosome: | chromosome 12 |
| Location: | 6279652 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g537150 | (1 of 20) IPR008972 - Cupredoxin | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTACCGGTATGCATGGAGGACACGCACACA |
| Internal bar code: | GTGTGGAATGGTGCCTGGGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 672 |
| LEAP-Seq percent confirming: | 99.0828 |
| LEAP-Seq n confirming: | 3457 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGGCAGAGAAACTTGAGTC |
| Suggested primer 2: | TGCTTACTTGCATGGAGTCG |