| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.214727 |
| Chromosome: | chromosome 9 |
| Location: | 2111574 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393650 | (1 of 1) PF00069//PF01590 - Protein kinase domain (Pkinase) // GAF domain (GAF) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACTTCATACCGACCATTCTATTTGCACAG |
| Internal bar code: | TTACCCGGGGATACTCCGAGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 834 |
| LEAP-Seq percent confirming: | 97.9524 |
| LEAP-Seq n confirming: | 6793 |
| LEAP-Seq n nonconfirming: | 142 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCCCTACAGTGTACCCAGA |
| Suggested primer 2: | GATAAGAGCGTCAAGCGTCC |