Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.214740 |
Chromosome: | chromosome 17 |
Location: | 2997648 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre17.g720350 | ARC12,MINE1 | (1 of 1) PTHR33404:SF2 - CELL DIVISION TOPOLOGICAL SPECIFICITY FACTOR HOMOLOG, CHLOROPLASTIC; chloroplast division site-determinant MinE | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCCGTGGGGTCCCACTCCAGCACGACGC |
Internal bar code: | GCGGCACCGAGAGGGCTCGGCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 679 |
LEAP-Seq percent confirming: | 99.7714 |
LEAP-Seq n confirming: | 873 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AACGCATTCCTTCGCATATC |
Suggested primer 2: | CCAGCGCTTTCTCTGGTATC |