Insertion junction: LMJ.RY0402.214740_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.214740
Chromosome:chromosome 17
Location:2997648
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g720350 ARC12,MINE1 (1 of 1) PTHR33404:SF2 - CELL DIVISION TOPOLOGICAL SPECIFICITY FACTOR HOMOLOG, CHLOROPLASTIC; chloroplast division site-determinant MinE CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTCCGTGGGGTCCCACTCCAGCACGACGC
Internal bar code:GCGGCACCGAGAGGGCTCGGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:679
LEAP-Seq percent confirming:99.7714
LEAP-Seq n confirming:873
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:AACGCATTCCTTCGCATATC
Suggested primer 2:CCAGCGCTTTCTCTGGTATC