Insertion junction: LMJ.RY0402.214746_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.214746
Chromosome:chromosome 7
Location:6319216
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g357033 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCTCAGCACTGCCAGCCCCCGGCAGCCCC
Internal bar code:TCGAAGTGTCCCGGGAGAGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:304
LEAP-Seq percent confirming:67.6718
LEAP-Seq n confirming:1930
LEAP-Seq n nonconfirming:922
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:CACCCAACATAACACCCCAT
Suggested primer 2:ACACACACACGCACACACAC