| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.214750 |
| Chromosome: | chromosome 16 |
| Location: | 2308027 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g659250 | CYB5,CYB5-5 | (1 of 2) PTHR19359//PTHR19359:SF29 - CYTOCHROME B5 // SUBFAMILY NOT NAMED; Cytochrome b5 flavohemoprotein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTGGGTCACAGCCGAGACAGTACCACGA |
| Internal bar code: | GTCGCCGATGAACGGCGCTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 740 |
| LEAP-Seq percent confirming: | 99.0246 |
| LEAP-Seq n confirming: | 11371 |
| LEAP-Seq n nonconfirming: | 112 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCAGAAGCTTTCACCCGT |
| Suggested primer 2: | AGTGCAACTGTGCGGTAGTG |