Insertion junction: LMJ.RY0402.214766_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.214766
Chromosome:chromosome 9
Location:7076517
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g411525 (1 of 107) PF00069//PF07714 - Protein kinase domain (Pkinase) // Protein tyrosine kinase (Pkinase_Tyr) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTAGCAGACGCGTTGTCTGGGTTGTTGAA
Internal bar code:CACGCTGAGCGAGCACGGGTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1265
LEAP-Seq percent confirming:94.9387
LEAP-Seq n confirming:24235
LEAP-Seq n nonconfirming:1292
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:TAGTGACGGTACTGCGCAAC
Suggested primer 2:CAGAAGGGGCGTGTGTTTAT